Protein-coding gene in the species Homo sapiens
B3GAT2 |
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![](//upload.wikimedia.org/wikipedia/commons/thumb/4/42/Protein_B3GAT2_PDB_2d0j.png/250px-Protein_B3GAT2_PDB_2d0j.png) |
Available structures |
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PDB | Ortholog search: PDBe RCSB |
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Identifiers |
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Aliases | B3GAT2, GLCATS, beta-1,3-glucuronyltransferase 2 |
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External IDs | OMIM: 607497; MGI: 2389490; HomoloGene: 50574; GeneCards: B3GAT2; OMA:B3GAT2 - orthologs |
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Gene location (Human) |
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![Chromosome 6 (human)](//upload.wikimedia.org/wikipedia/commons/thumb/0/03/Ideogram_human_chromosome_6.svg/300px-Ideogram_human_chromosome_6.svg.png) | Chr. | Chromosome 6 (human)[1] |
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| Band | 6q13 | Start | 70,856,679 bp[1] |
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End | 70,957,060 bp[1] |
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Gene location (Mouse) |
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![Chromosome 1 (mouse)](//upload.wikimedia.org/wikipedia/commons/thumb/f/fb/Ideogram_house_mouse_chromosome_1.svg/260px-Ideogram_house_mouse_chromosome_1.svg.png) | Chr. | Chromosome 1 (mouse)[2] |
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| Band | 1|1 A5 | Start | 23,800,834 bp[2] |
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End | 23,888,239 bp[2] |
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RNA expression pattern |
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Bgee | Human | Mouse (ortholog) |
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Top expressed in | - ventricular zone
- entorhinal cortex
- ganglionic eminence
- buccal mucosa cell
- pons
- Brodmann area 46
- postcentral gyrus
- hippocampus proper
- endothelial cell
- corpus callosum
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| Top expressed in | - proximal tubule
- right kidney
- ventricular zone
- urethra
- human kidney
- zygote
- dentate gyrus of hippocampal formation granule cell
- primary visual cortex
- superior frontal gyrus
- neural tube
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| More reference expression data |
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BioGPS | |
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Gene ontology |
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Molecular function | - transferase activity
- metal ion binding
- galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity
| Cellular component | - integral component of membrane
- Golgi apparatus
- membrane
- Golgi membrane
| Biological process | - glycosaminoglycan metabolic process
- protein glycosylation
- chondroitin sulfate proteoglycan biosynthetic process
- chondroitin sulfate metabolic process
- carbohydrate metabolic process
| Sources:Amigo / QuickGO |
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Orthologs |
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Species | Human | Mouse |
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Entrez | | |
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Ensembl | | |
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UniProt | | |
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RefSeq (mRNA) | | |
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RefSeq (protein) | | |
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Location (UCSC) | Chr 6: 70.86 – 70.96 Mb | Chr 1: 23.8 – 23.89 Mb |
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PubMed search | [3] | [4] |
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Wikidata |
View/Edit Human | View/Edit Mouse |
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Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 is an enzyme that in humans is encoded by the B3GAT2 gene.[5][6]
The product of this gene is a transmembrane protein belonging to the glucuronyltransferase family, and catalyzes the transfer of a beta-1,3 linked glucuronic acid to a terminal galactose in different glycoproteins or glycolipids containing a Gal-beta-1-4GlcNAc or Gal-beta-1-3GlcNAc residue. The encoded protein is involved in the synthesis of the human natural killer-1 (HNK-1) carbohydrate epitope, a sulfated trisaccharide implicated in cellular migration and adhesion in the nervous system.[6]
Use of HNK-1 Antibody for Neural Crest Research
Antibodies raised against the HNK-1 epitope have played a large role in studies of the neural crest, especially in the avian embryo.[7] The first antibody raised against this epitope was NC-1,[8] which permitted much easier analyses of neural crest migration pathways. In avians, and especially in other vertebrates, the results of HNK-1 staining should be interpreted with caution as the epitope is not unique to the neural crest.
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000112309 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026156 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Imiya K, Ishizaki T, Seiki T, Saito F, Inazawa J, Oka S, Kawasaki T (Oct 2002). "cDNA cloning, genomic structure and chromosomal mapping of the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope". Gene. 296 (1–2): 29–36. doi:10.1016/S0378-1119(02)00840-5. PMID 12383500.
- ^ a b "Entrez Gene: B3GAT2 beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase S)".
- ^ Bronner-Fraser M (May 1986). "Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-1". Dev. Biol. 115 (1): 44–55. doi:10.1016/0012-1606(86)90226-5. PMID 3516760.
- ^ Vincent M, Thiery JP (June 1984). "A cell surface marker for neural crest and placodal cells: further evolution in peripheral and central nervous system". Dev. Biol. 103 (2): 468–81. doi:10.1016/0012-1606(84)90334-8. PMID 6202575.
External links
- Human B3GAT2 genome location and B3GAT2 gene details page in the UCSC Genome Browser.
- PDBe-KB provides an overview of all the structure information available in the PDB for Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 (B3GAT2)
Further reading
- Nagase T, Kikuno R, Ohara O (2002). "Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins". DNA Res. 8 (6): 319–27. doi:10.1093/dnares/8.6.319. PMID 11853319.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Marcos I, Galán JJ, Borrego S, Antiñolo G (2003). "Cloning, characterization, and chromosome mapping of the human GlcAT-S gene". J. Hum. Genet. 47 (12): 677–80. doi:10.1007/s100380200103. PMID 12522689.
- Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6". Nature. 425 (6960): 805–11. Bibcode:2003Natur.425..805M. doi:10.1038/nature02055. PMID 14574404.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Kakuda S, Oka S, Kawasaki T (2004). "Purification and characterization of two recombinant human glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate in Escherichia coli". Protein Expr. Purif. 35 (1): 111–9. doi:10.1016/j.pep.2003.12.021. PMID 15039073.
- Kakuda S, Sato Y, Tonoyama Y, et al. (2005). "Different acceptor specificities of two glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate". Glycobiology. 15 (2): 203–10. doi:10.1093/glycob/cwi001. PMID 15470230.
- Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
- Shiba T, Kakuda S, Ishiguro M, et al. (2006). "Crystal structure of GlcAT-S, a human glucuronyltransferase, involved in the biosynthesis of the HNK-1 carbohydrate epitope". Proteins. 65 (2): 499–508. doi:10.1002/prot.21118. PMID 16897771. S2CID 31290955.
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2.4.1: Hexosyl- transferases | |
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2.4.2: Pentosyl- transferases | |
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2.4.99: Sialyl transferases | |
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